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Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie
Achim Thomzig, … , Jessica Mai, Michael Beekes
Achim Thomzig, … , Jessica Mai, Michael Beekes
Published May 15, 2004
Citation Information: J Clin Invest. 2004;113(10):1465-1472. https://doi.org/10.1172/JCI21083.
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Categories: Article Neuroscience

Preclinical deposition of pathological prion protein PrPSc in muscles of hamsters orally exposed to scrapie

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Abstract

Recently, pathological prion protein PrPSc, the putative key constituent of infectious agents causing transmissible spongiform encephalopathies (TSEs), was found in muscles of rodents experimentally infected with scrapie and in patients with Creutzfeldt-Jakob disease (CJD). For the assessment of risk scenarios originating from these findings (e.g., alimentary transmission of pathogens associated with bovine spongiform encephalopathy [BSE] and chronic wasting disease [CWD] via tainted beef and game or iatrogenic dissemination of CJD agent through contaminated surgical instruments) more detailed information about the time course of PrPSc accumulation in muscles at preclinical and clinical stages of incubation is needed. Here we show that PrPSc in muscles of hamsters fed with scrapie can be detected prior to the onset of clinical symptoms, but that the bulk of PrPSc was deposited late in clinical disease. Additionally, regarding the question of how muscles become invaded, we report on the intramuscular location of PrPSc and substantial indications for centrifugal spread of infection from spinal motor neurons to myofibers. Our findings in a well-established animal model for TSEs contribute to a better assessment of the risks for public health emanating from “Prions in skeletal muscle” and provide new insights into the pathophysiological spread of TSE agents through the body.

Authors

Achim Thomzig, Walter Schulz-Schaeffer, Christine Kratzel, Jessica Mai, Michael Beekes

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