Daf1–/– and Daf1+/+ mice were injected with McAb-3 24 hours prior to videotaping (photographs shown were acquired from video images). (a) The Daf1–/– mouse had a hunched posture and could not extend its digits. Its hind limbs could not support its weight. This mouse had a hang time of less than 10 seconds. (b) The Daf1+/+ littermate was normally active (shadow under belly, turning body), showing normal posture and leg position. It had a hang time of more than 8 minutes.