Immunoreactive HSP-70 levels in lung homogenate. (a) Representative immunoblot. Homogenate was obtained 48 hours after virus administration and operative intervention. Ten micrograms of homogenate were loaded per lane. Primary antibody was polyclonal goat anti-rat HSP-70; secondary antibody was rabbit anti-goat IgG. Detection was with enhanced chemiluminescence. Lane 1, purified HSP-70 (positive control); lane 2, unoperated control without AdHSP; lane 3, unoperated control with AdHSP, lane 4, sham operation without AdHSP; lane 5, sham operation with AdHSP; lane 6, 2CLP without AdHSP; lane 7, 2CLP with AdHSP; lane 8, 2CLP with AdGFP. (b) Graphic representation of densitometric quantification of HSP-70 immunoblots. n = 3 for each intervention. *Significantly different (P < 0.05) from value without virus administration. Values are arbitrary densitometric units, expressed as mean ± SD. (c) Graphic representation of densitometric quantification of AdHSP and AdGFP expression over time following sham operation. Values are mean ± SD of densitometric measurements made on three immunoblots. *Significantly different (P < 0.05) from all other measurements. White bars, no virus; black bars, AdHSP, gray bars, AdGFP.