Mark W. Musch, Lane L. Clarke, Daniel Mamah, Lara R. Gawenis, Zheng Zhang, William Ellsworth, David Shalowitz, Navdha Mittal, Petros Efthimiou, Ziad Alnadjim, Steve D. Hurst, Eugene B. Chang, Terrence A. Barrett
(a) Time course of effect of anti-CD3 mAb on jejunal wt/l ratio. Mice were injected with 0.2 mg anti-CD3 mAb at time 0. Loop wt/l ratios were measured after varying times. Values are means ± SE for six determinations. (b) Effect of anti-TNF and anti–IFN-γ antibodies on jejunal wt/l ratios at 3 hours. Mice were injected simultaneously with 0.2 mg control or anti-CD3 mAb along with neutralizing mAb to TNF or IFN-γ. (c) Control or anti-CD3 mAb was injected in C57BL/6 or in TNFR-1 or IFN-γ knockout mice. Weight-to-length ratios were determined after 3 hours. Values are means ± SE for six determinations in each group. In all cases, *P < 0.05, **P < 0.01 by comparison using ANOVA. In (a), all comparisons were with 0-time control. In (b) and (c), control mAb-treated, anti–CD3-stimulated mice were initially compared with control mAb-treated, unstimulated controls. In (b), results in anti-TNF mAb and anti–IFN-γ–treated, anti–CD3-stimulated mice were compared with control mAb-treated, anti–CD3-stimulated mice. In (c), results in anti–CD3-stimulated TNFR-1–/– and IFN-γ–/– mice were compared with anti–CD3-stimulated B6 mice. The data indicate that TNF but not IFN-γ inhibition prevented anti–CD3-induced diarrhea.