Complex phenotype of mice lacking occludin, a component of tight junction strands

M Saitou, M Furuse, H Sasaki… - Molecular biology of …, 2000 - Am Soc Cell Biol
M Saitou, M Furuse, H Sasaki, JD Schulzke, M Fromm, H Takano, T Noda, S Tsukita
Molecular biology of the cell, 2000Am Soc Cell Biol
Occludin is an integral membrane protein with four transmembrane domains that is
exclusively localized at tight junction (TJ) strands. Here, we describe the generation and
analysis of mice carrying a null mutation in the occludin gene. Occludin−/− mice were born
with no gross phenotype in the expected Mendelian ratios, but they showed significant
postnatal growth retardation. Occludin−/− males produced no litters with wild-type females,
whereas occludin−/− females produced litters normally when mated with wild-type males but …
Occludin is an integral membrane protein with four transmembrane domains that is exclusively localized at tight junction (TJ) strands. Here, we describe the generation and analysis of mice carrying a null mutation in the occludin gene. Occludin −/− mice were born with no gross phenotype in the expected Mendelian ratios, but they showed significant postnatal growth retardation. Occludin −/− males produced no litters with wild-type females, whereas occludin −/− females produced litters normally when mated with wild-type males but did not suckle them. In occludin −/− mice, TJs themselves did not appear to be affected morphologically, and the barrier function of intestinal epithelium was normal as far as examined electrophysiologically. However, histological abnormalities were found in several tissues, i.e., chronic inflammation and hyperplasia of the gastric epithelium, calcification in the brain, testicular atrophy, loss of cytoplasmic granules in striated duct cells of the salivary gland, and thinning of the compact bone. These phenotypes suggested that the functions of TJs as well as occludin are more complex than previously supposed.
Am Soc Cell Biol