[HTML][HTML] Rapid susceptibility testing for herpes simplex virus type 1 using real-time PCR
MT van der Beek, ECJ Claas, CS van der Blij-de… - Journal of clinical …, 2013 - Elsevier
BACKGROUND: Susceptibility testing of herpes simplex virus type 1 (HSV-1) is traditionally
performed by a plaque reduction assay (PRA), but this is labor intensive, time consuming
and has a manual read out. OBJECTIVES: The goal of this study was to develop an
internally controlled real time PCR-based phenotypical susceptibility test for HSV-1 that is
suitable for use in a clinical diagnostic setting. STUDY DESIGN: A DNA reduction assay
(DRA) was developed and validated on a test panel of 26 well-characterized isolates of …
performed by a plaque reduction assay (PRA), but this is labor intensive, time consuming
and has a manual read out. OBJECTIVES: The goal of this study was to develop an
internally controlled real time PCR-based phenotypical susceptibility test for HSV-1 that is
suitable for use in a clinical diagnostic setting. STUDY DESIGN: A DNA reduction assay
(DRA) was developed and validated on a test panel of 26 well-characterized isolates of …
