Mechanisms at the cellular level during induction of high zone tolerance in vitro
E Diener, M Feldmann - Cellular immunology, 1972 - Elsevier
The in vitro induction of high zone tolerance to polymerized flagellin of S. adelaide in CBA
mouse spleen cells consists of two stages:(a) Attachment of tolerance inducing
concentrations of antigen to the surface of immunocompetent cells.(b) Inactivation of the
immunocompetent cells by the surface-bound antigen. The definition of these two stages is
based on the finding that trypsin treatment of spleen cells within 16 hr after tolerance
induction in vitro completely reverses the state of unresponsiveness. However, reversibility …
mouse spleen cells consists of two stages:(a) Attachment of tolerance inducing
concentrations of antigen to the surface of immunocompetent cells.(b) Inactivation of the
immunocompetent cells by the surface-bound antigen. The definition of these two stages is
based on the finding that trypsin treatment of spleen cells within 16 hr after tolerance
induction in vitro completely reverses the state of unresponsiveness. However, reversibility …