Medullary thymic epithelial cells and CD8α+ dendritic cells coordinately regulate central tolerance but CD8α+ cells are dispensable for thymic regulatory T cell …

O Herbin, AJ Bonito, S Jeong, EG Weinstein… - Journal of …, 2016 - Elsevier
O Herbin, AJ Bonito, S Jeong, EG Weinstein, AH Rahman, H Xiong, M Merad
Journal of autoimmunity, 2016Elsevier
In the thymus, antigen presenting cells (APCs) namely, medullary thymic epithelial cells
(mTECs) and thymic dendritic cells (tDCs) regulate T cell tolerance through elimination of
autoreactive T cells and production of thymic T regulatory (tTreg) cells. How the different
APCs in the thymus share the burden of tolerazing the emerging T cell repertoire remains
unclear. For example, while mutations that inhibit mTEC development or function associate
with peripheral autoimmunity, the role of tDCs in organ-specific autoimmunity and tTreg cell …
Abstract
In the thymus, antigen presenting cells (APCs) namely, medullary thymic epithelial cells (mTECs) and thymic dendritic cells (tDCs) regulate T cell tolerance through elimination of autoreactive T cells and production of thymic T regulatory (tTreg) cells. How the different APCs in the thymus share the burden of tolerazing the emerging T cell repertoire remains unclear. For example, while mutations that inhibit mTEC development or function associate with peripheral autoimmunity, the role of tDCs in organ-specific autoimmunity and tTreg cell production remains controversial. In this report we used mice depleted of mTECs and/or CD8α+ DCs, to examine the contributions of these cell populations in thymic tolerance. We found that while mice depleted of CD8α+ DCs or mTECs were normal or developed liver inflammation respectively, combined depletion of mTECs and CD8α+ DCs resulted in overt peripheral autoimmunity. The autoimmune manifestations in mice depleted of both mTECs and CD8α+ cDCs associated with increased percentages of CD4+ and CD8+ T cells in the thymus. In contrast, while mTEC depletion resulted in reduced percentages of tTreg cells, no additional effect was observed when CD8α+ DCs were also depleted. These results reveal that: 1) mTECs and CD8α+ DCs cooperatively safeguard against peripheral autoimmunity through thymic T cell deletion; 2) CD8α+ DCs are dispensable for tTreg cell production, whereas mTECs play a non-redundant role in this process; 3) mTECs and CD8α+ DCs make unique contributions to tolerance induction that cannot be compensated for by other thymic APCs such as migratory SIRPα+ or plasmacytoid DCs.
Elsevier