Cloning and expression of the human erythropoietin gene.
FK Lin, S Suggs, CH Lin, JK Browne… - Proceedings of the …, 1985 - National Acad Sciences
FK Lin, S Suggs, CH Lin, JK Browne, R Smalling, JC Egrie, KK Chen, GM Fox, F Martin…
Proceedings of the National Academy of Sciences, 1985•National Acad SciencesThe human erythropoietin gene has been isolated from a genomic phage library by using
mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is
contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening
sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid
signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The
erythropoietin gene, when introduced into Chinese hamster ovary cells, produces …
mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is
contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening
sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid
signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The
erythropoietin gene, when introduced into Chinese hamster ovary cells, produces …
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.
National Acad Sciences