[HTML][HTML] Transactivation and inhibitory domains of hypoxia-inducible factor 1α: modulation of transcriptional activity by oxygen tension
BH Jiang, JZ Zheng, SW Leung, R Roe… - Journal of Biological …, 1997 - ASBMB
Hypoxia-inducible factor 1 (HIF-1) binds to cis-acting hypoxia-response elements within the
erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in
hypoxic cells. HIF-1 is a basic helix-loop-helix transcription factor composed of HIF-1α and
HIF-1β subunits. Here, we demonstrate that HIF-1α contains two transactivation domains
located between amino acids 531 and 826. When expressed as GAL4 fusion proteins, the
transcriptional activity of these domains increased in response to hypoxia. Fusion protein …
erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in
hypoxic cells. HIF-1 is a basic helix-loop-helix transcription factor composed of HIF-1α and
HIF-1β subunits. Here, we demonstrate that HIF-1α contains two transactivation domains
located between amino acids 531 and 826. When expressed as GAL4 fusion proteins, the
transcriptional activity of these domains increased in response to hypoxia. Fusion protein …