Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex
U Hibner, BM Alberts - Nature, 1980 - nature.com
U Hibner, BM Alberts
Nature, 1980•nature.comMore than 50 copies of a ΦX174 DNA template can be made in 60 min in an in vitro DNA
replication system consisting of seven purified replication proteins isolated from T4
bacteriophage-infected cells. By transfecting with the DNA products and assaying for the
reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is
revealed; the in vitro system is also shown to respond to the mutagenic effect of Mn2+ and to
display strong base-pair context effects on fidelity, as expected from in vivo studies.
replication system consisting of seven purified replication proteins isolated from T4
bacteriophage-infected cells. By transfecting with the DNA products and assaying for the
reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is
revealed; the in vitro system is also shown to respond to the mutagenic effect of Mn2+ and to
display strong base-pair context effects on fidelity, as expected from in vivo studies.
Abstract
More than 50 copies of a ΦX174 DNA template can be made in 60 min in an in vitro DNA replication system consisting of seven purified replication proteins isolated from T4 bacteriophage-infected cells. By transfecting with the DNA products and assaying for the reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is revealed; the in vitro system is also shown to respond to the mutagenic effect of Mn2+ and to display strong base-pair context effects on fidelity, as expected from in vivo studies.
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