Novel inhalational murine model of invasive pulmonary aspergillosis
DC Sheppard, G Rieg, LY Chiang… - Antimicrobial agents …, 2004 - Am Soc Microbiol
Antimicrobial agents and chemotherapy, 2004•Am Soc Microbiol
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease.
Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then
infected in an aerosol chamber. This procedure reproducibly delivered 1× 103 to 3× 103
conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by
amphotericin B.
Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then
infected in an aerosol chamber. This procedure reproducibly delivered 1× 103 to 3× 103
conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by
amphotericin B.
Abstract
We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol chamber. This procedure reproducibly delivered 1 × 103 to 3 × 103 conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by amphotericin B.
American Society for Microbiology