Interruption of cenph causes mitotic failure and embryonic death, and its haploinsufficiency suppresses cancer in zebrafish
X Zhao, L Zhao, T Tian, Y Zhang, J Tong… - Journal of biological …, 2010 - ASBMB
Kinetochore proteins associate with centromeric DNA and spindle microtubules and play
essential roles in chromosome segregation during mitosis. In this study, we uncovered a
zebrafish mutant, stagnant and curly (stac), that carries the Tol2 transposon element inserted
at the kinetochore protein H (cenph) locus. Mutant embryos exhibit discernible cell death as
early as 20 hours postfertilization, extensive apoptosis, and upward curly tail during the
pharyngula period and deform around 5 days postfertilization. The stac mutant phenotype …
essential roles in chromosome segregation during mitosis. In this study, we uncovered a
zebrafish mutant, stagnant and curly (stac), that carries the Tol2 transposon element inserted
at the kinetochore protein H (cenph) locus. Mutant embryos exhibit discernible cell death as
early as 20 hours postfertilization, extensive apoptosis, and upward curly tail during the
pharyngula period and deform around 5 days postfertilization. The stac mutant phenotype …