Redox regulation of lipopolysaccharide (LPS)-induced interleukin-8 (IL-8) gene expression mediated by NFκB and AP-1 in human astrocytoma U373 cells
C Tanaka, H Kamata, H Takeshita, H Yagisawa… - Biochemical and …, 1997 - Elsevier
C Tanaka, H Kamata, H Takeshita, H Yagisawa, H Hirata
Biochemical and biophysical research communications, 1997•ElsevierLPS-induced expression of the IL-8 gene was markedly enhanced by H2O2or by deprivation
of the cellular antioxidant glutathione by L-buthionine-(S, R)-sulfoximine (BSO) in human
astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-
cysteine (NAC) and other antioxidants. Transient expression analysis using the
chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by
LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation …
of the cellular antioxidant glutathione by L-buthionine-(S, R)-sulfoximine (BSO) in human
astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-
cysteine (NAC) and other antioxidants. Transient expression analysis using the
chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by
LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation …
LPS-induced expression of the IL-8 gene was markedly enhanced by H2O2or by deprivation of the cellular antioxidant glutathione by L-buthionine-(S,R)-sulfoximine (BSO) in human astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-cysteine (NAC) and other antioxidants. Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation of both NFκB and AP-1 was enhanced by BSO and inhibited by NAC. These results suggest that LPS-induced IL-8 gene expression is regulated by cellular redox via modulation of these transcription factors.
Elsevier
